检测人前列腺癌中17β一羟基类固醇脱氢酶7表达量的试剂盒

Kit for detecting expression level of 17 beta-hydroxysteroid dehydrogenase 7 in human prostate cancer

Abstract

The invention belongs to the field of biotechnology and relates to a real-time fluorescent quantitative PCR kit for detecting the mRNA expression level of 17 beta-hydroxysteroid dehydrogenase 7 (17 beta-HSD7) in human prostate cancer samples. The kit contains 2 * reverse transcription reaction solution, 20 * reverse transcriptase, water treated by diethyl pyrocarbonate, 2 * polymerase chain reaction solution, 20 * primers and fluorescent probes for detection and a standard product. The invention establishes a method for utilizing the Taqman technology for detecting the expression of the 17 beta-HSD7, and the detection practices prove that the kit and the detection method are feasible. As the method adopts the PCR amplification technology, the method can greatly improve the detection sensitivity of the 17 beta-HSD7 and obtain enough information in a very small number of samples; due to the application of the fluorescent probes, the specificity is also greatly improved and the false positive rate of the conventional PCR amplification is reduced.
本发明属于生物技术领域,涉及一种检测人前列腺癌样本中17β一羟基类固醇脱氢酶7(17β-HSD7)mRNA表达量的实时荧光定量PCR试剂盒。本试剂盒含有2×逆转录反应液,20×逆转录酶,焦碳酸乙二酯处理的水,2×聚合酶链反应液,20×检测用引物、荧光探针,标准品。本发明建立了利用Taqman技术检测17β-HSD7表达的方法,并经检测实践表明,本发明试剂盒及检测方法切实可行。由于本方法采用了PCR扩增技术,使得17β-HSD7表达的检测敏感性大大提高,可以在极少的标本中获得足够的信息,由于荧光探针的应用使得特异性也大大提高,降低了常规PCR扩增的假阳性率。

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NO-Patent Citations (2)

    Title
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